Plasmid
Part:BBa_K4181001:Design
Designed by: Junzhi Yan Group: iGEM22_SJTU-BioX-Shanghai (2022-09-18)
A transforming plasmid of Bacillus subtilis
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 653
Illegal XbaI site found at 815
Illegal XbaI site found at 846
Illegal XbaI site found at 5395
Illegal SpeI site found at 5491
Illegal PstI site found at 659
Illegal PstI site found at 3650
Illegal PstI site found at 6846 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 653
Illegal SpeI site found at 5491
Illegal PstI site found at 659
Illegal PstI site found at 3650
Illegal PstI site found at 6846 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 653
Illegal BamHI site found at 635
Illegal XhoI site found at 5745 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 653
Illegal XbaI site found at 815
Illegal XbaI site found at 846
Illegal XbaI site found at 5395
Illegal SpeI site found at 5491
Illegal PstI site found at 659
Illegal PstI site found at 3650
Illegal PstI site found at 6846 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 653
Illegal XbaI site found at 815
Illegal XbaI site found at 846
Illegal XbaI site found at 5395
Illegal SpeI site found at 5491
Illegal PstI site found at 659
Illegal PstI site found at 3650
Illegal PstI site found at 6846
Illegal NgoMIV site found at 5709 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 6672
Illegal SapI.rc site found at 1433
Design Notes
If you want to insert the target gene, be sure to insert the gene between the two amyE sites.
Source
Artificially constructed plasmids