Plasmid

Part:BBa_K4181001:Design

Designed by: Junzhi Yan   Group: iGEM22_SJTU-BioX-Shanghai   (2022-09-18)


A transforming plasmid of Bacillus subtilis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 653
    Illegal XbaI site found at 815
    Illegal XbaI site found at 846
    Illegal XbaI site found at 5395
    Illegal SpeI site found at 5491
    Illegal PstI site found at 659
    Illegal PstI site found at 3650
    Illegal PstI site found at 6846
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 653
    Illegal SpeI site found at 5491
    Illegal PstI site found at 659
    Illegal PstI site found at 3650
    Illegal PstI site found at 6846
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 653
    Illegal BamHI site found at 635
    Illegal XhoI site found at 5745
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 653
    Illegal XbaI site found at 815
    Illegal XbaI site found at 846
    Illegal XbaI site found at 5395
    Illegal SpeI site found at 5491
    Illegal PstI site found at 659
    Illegal PstI site found at 3650
    Illegal PstI site found at 6846
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 653
    Illegal XbaI site found at 815
    Illegal XbaI site found at 846
    Illegal XbaI site found at 5395
    Illegal SpeI site found at 5491
    Illegal PstI site found at 659
    Illegal PstI site found at 3650
    Illegal PstI site found at 6846
    Illegal NgoMIV site found at 5709
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 6672
    Illegal SapI.rc site found at 1433


Design Notes

If you want to insert the target gene, be sure to insert the gene between the two amyE sites.


Source

Artificially constructed plasmids

References